Secretariat, Alumni Association, IDAC | |
Date | Tuesday, 21 January 2025, 17:00~ |
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Room | 7th floor, Seminar Room (1), IDAC Center for Aging Research |
Title | Structure and dynamics of molecular machineries regulating mitochondrial fission and fusion |
Speaker | Yuhei Araiso |
Affiliation | Division of Health Sciences, Graduate School of Medicine, Kanazawa University |
Organizer | Haruna Tani(Dept.Modomics Biology and Medicine, ext 8569) |
Abstract | Mitochondrial homeostasis is critical for living cells and closely related to their morphologies. Their shape is finely tuned by molecular machineries controlling the membrane fission and fusion. Dynamin-related protein 1 (Drp1) is an intracellular GTPase that regulates mitochondrial fission. Drp1 is recruited to the mitochondrial outer membrane. Upon accumulating on the membrane surface, Drp1 oligomerizes to encircle along the mitochondrial outer membrane. Then, the Drp1 oligomer is thought to drive membrane fission depending on the GTP hydrolysis. To elucidate the precise fission mechanism, we are attempting the real-time imaging of the molecular dynamics of Drp1 by high-speed atomic force microscopy (HS-AFM). The HS-AFM imaging clearly captured that the Drp1 dimer is recruited and forms an oligomer encircling the phospholipid membrane with GTP-dependent manner. |