Secretariat, Alumni Association, IDAC
Date Tuesday, 21 January 2025, 17:00~
Room 7th floor, Seminar Room (1), IDAC Center for Aging Research
Title Structure and dynamics of molecular machineries regulating mitochondrial fission and fusion
Speaker Yuhei Araiso
Affiliation Division of Health Sciences, Graduate School of Medicine, Kanazawa University
Organizer Haruna Tani(Dept.Modomics Biology and Medicine, ext 8569)
Abstract Mitochondrial homeostasis is critical for living cells and closely related to their morphologies. Their shape is finely tuned by molecular machineries controlling the membrane fission and fusion. Dynamin-related protein 1 (Drp1) is an intracellular GTPase that regulates mitochondrial fission. Drp1 is recruited to the mitochondrial outer membrane. Upon accumulating on the membrane surface, Drp1 oligomerizes to encircle along the mitochondrial outer membrane. Then, the Drp1 oligomer is thought to drive membrane fission depending on the GTP hydrolysis.
To elucidate the precise fission mechanism, we are attempting the real-time imaging of the molecular dynamics of Drp1 by high-speed atomic force microscopy (HS-AFM). The HS-AFM imaging clearly captured that the Drp1 dimer is recruited and forms an oligomer encircling the phospholipid membrane with GTP-dependent manner.