| 日時： | 令和元年10月25日（金）午後5時〜午後6時30分 |
| 場所： | 加齢研実験研究棟7階 セミナー室 1 |
| 演題： | High-resolution Analysis of Transcribed Enhancers Using a Novel 5’ Nascent RNA Sequencing |
| 講師： | 村川 泰裕 |
| 所属： | 理化学研究所 生命医科学研究センター |
| 担当： | 本橋 ほづみ（所属 遺伝子発現制御分野・内線8550） |
| 要旨： | Enhancers are activated in a highly cell-type specific manner, and play key roles in generating cell-type-specific transcriptomes. Capped transcripts are generated bi-directionally from active enhancers, but these transcripts are actively degraded by the nuclear exosome complex immediately after their synthesis. Here we developed a simple and robust NET-CAGE technology to profile 5’-ends of nascent RNAs in diverse cells and tissues, and we studied RNA synthesis and degradation at the transcription start site level. Sensitive and high-resolution analysis of enhancer-derived RNAs without the influence of RNA degradation allowed us to identify a number of new enhancers at an unprecedented depth, and to delineate primary locations of transcription within super-enhancers. I will talk about new insights into the dynamic and topological interplay between cis-regulatory elements. |
| Reference: | Hirabayashi S., Bhagat S., Matsuki Y., Takegami Y., Uehata T., Kanemaru A., Itoh M., Shirakawa K., Takaori-Kondo A., Takeuchi O., Carninci P., Katayama S., Hayashizaki Y., Kere J., Kawaji H., Murakawa Y. NET-CAGE Characterizes Dynamics and Topology of Human Transcribed Cis-regulatory Elements. Nature Genetics, 51(9):1369-1379 (2019) |
