IDAC Fellow

 

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IDAC Fellow Research Group: Chromatin Remodeling and DNA Repair in Aging and Cancer Therapy (Former Division of Dynamic Proteome).

This research group aims at the elucidation of DNA repair and chromatin remodeling in aging and cancer and its application to cancer therapy by means of external grants and donations. We furthermore apply our original proteomics technology to the collaboration with internal and international researchers. Officially this research group belongs to the department of molecular oncology of IDAC.

The following diagram (figure 1) shows our working hypothesis based on our and other results:

 

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PUBLICATIONS OF THIS LAB:

1. Ui A, and Yasui A. Collaboration of MLLT1/ENL, polycomb and ATM for transcription and genome integrity. Nucleus. 7;138-45, 2016
2. Ui A, Nagaura Y., and Yasui A. Transcriptional elongation factor ENL phosphorylated by ATM recruits Polycomb and switches off transcription for DSB repair. Mol Cell. 58:468-82, 2015
3. Tokuyama Y, Furusawa Y, Ide H, Yasui A, Terato H. Role of isolated and clustered DNA damage and the post-irradiating repair process in the effects of heavy ion beam irradiation. J Radiat Res. 2015 May;56(3):446-55. doi: 10.1093/jrr/rru122. Epub 2015 Feb 25.
4. Homma Y, Kanno S, Sasaki K, Nishita M, Yasui A, Asano T, Ohashi K, Mizuno K. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem. Sep 19;289(38):26302-13 2014
5. Watanabe R, Ui A, Kanno S-I, Ogiwara H, Nagase T, Kohno T, and Yasui A. SWI/SNF factors required for cellular resistance to DNA damage include ARID1A and ARID1B and show interdependent protein stability, Cancer Res. 74:2465-2475, 2014.
6. Ui A, Ogiwara H, Nakajima S, Kanno S-I, Watanabe R, Harata M, Okayama H, Harris CC, Yokota J, Yasui A and Kohno T. Possible involvement of LKB1-AMPK signaling in non-homologous end joining. Oncogen 33, 1640-1648, 2014.
7. Nakajima S, Lan L, Wei L, Hsieh CL, Rapić-Otrin V, Yasui A, Levine AS. Ubiquitin-specific protease 5 is required for the efficient repair of DNA double-strand breaks. PLoS One. 9, 2014.
8. Kato K, Nakajima K, Ui A, Muto-Terao Y, Ogiwara H, Nakada S. Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice. Mol Cell. 2014 Feb 20;53(4):617-30.
9. Zaytseva O, Tenis N, Mitchell N, Kanno S, Yasui A, Heierhorst J, Quinn LM. The novel zinc finger protein dASCIZ regulates mitosis in Drosophila via an essential role in dynein light-chain expression. Genetics. 196, 443-53, 2014.
10. Lan L, Nakajima S, Wei L, Sun L, Hsieh CL, Sobol RW, Bruchez M, Van Houten B, Yasui A, Levine AS. Novel method for site-specific induction of oxidative DNA damage reveals differences in recruitment of repair proteins to heterochromatin and euchromatin. Nucleic Acids Res. 42, 2330-45, 2014.
11. Matsuzawa A, Kanno S, Nakayama M, Mochiduki H, Wei L, Shimaoka T, Furukawa Y, Kato K, Shibata S, Yasui A, Ishioka C, Chiba N. The BRCA1/BARD1-interacting protein OLA1 functions in centrosome regulation. Mol Cell, 53, 101-14, 2014.
12. Wei L, Nakajima S, Hsieh CL, Kanno S, Masutani M, Levine AS, Yasui A, and Lan L. Damage response of XRCC1 at sites of DNA single strand breaks is regulated by phosphorylation and ubiquitylation after degradation of poly(ADP-ribose). J Cell Sci. 126, 4414-23, 2013.
13. Ogiwara H, Ui A, Shiotani B, Zou L, Yasui A, and Kohno T. Curcumin suppresses multiple DNA damage response pathways and has potency as a sensitizer to PARP inhibitor. Carcinogenesis, 34, 2486-97, 2013.
14. Toga T, Kuraoka I, Yasui A, and Iwai S. A transfection reporter for the prevention of false-negative results in molecular beacon experiments. Anal Biochem. 440, 9-11, 2013.
15. Yasui A. Alternative excision repair pathways, Cold Spring Harb. Perspect. Biol. 6, 2013
16. Itoh G, Sugino S, Ikeda M, Mizuguchi M, Kanno SI, Amin MA, Iemura K, Yasui A, Hirota T, and Tanaka K., Nucleoporin Nup188 is required for chromosome alignment in mitosis. Cancer Sci. 104, 871-879, 2013.
17. Hong Z, Jiang J, Ma J, Dai S, Xu T, Li H, and Yasui A. The role of hnRPUL1 involved in DNA damage response is related to PARP1. PLoS One, 8, 2013.
18. Yang Y, Wang C, Zhang P, Gao K, Wang D, Yu H, Zhang T, Jiang S, Hexige S, Hong Z, Yasui A, Liu JO, Huang H, Yu L. Polycomb group protein PHF1 regulates p53-dependent cell growth arrest and apoptosis. J. Biol. Chem. 288, 529-539, 2013
19. Musselman CA, Avvakumov N, Watanabe R, Abraham G, Allen C, Roy S, Nunez J, Nickolof J, Kulesza CA, Yasui A, Cote J and Kutateladze TG. Molecular basis for H3K36me3 recognition by the tudor domains of PHF1. Nat. Struct. Mol Biol. 19, 1266-1272, 2012.
20. Zhang X, Horibata K, Saijo M, Ishigami C, Ukai A, Kanno S, Tahara H, Neilan EG, Honma M, Nohmi T, Yasui A, and Tanaka K. Mutations in UVSSA cause UV-sensitive syndrome and destabilize ERCC6 in transcription-coupled DNA repair.. Nat Genet. 44, 593-597, 2012.
21. Nomura H, Yoshimura A, Edo T, Kanno S, Tada S, Seki M, Yasui A, Enomoto T. WRNIP1 accumulates at laser light irradiated sites rapidly via its ubiquitin-binding zinc finger domain and independently from its ATPase domain. Biochem Biophys Res Commun. 417, 1145-1150, 2012.
22. Wei, L. Lan L, Yasui A, Tanaka K, Saijo M, Matsuzawa A, Kashiwagi R, Maseki E, Hu Y, Parvin JD, Ishioka C, and Chiba N. BRCA1 contributes to transcription-coupled repair of DNA damage through polyubiquitination and degradation of Cockayne syndrome B protein. Cancer Sci. 102, 1840-1847, 2011.
23. Li S, Kanno SI, Watanabe R, Ogiwara H, Kohno T, Watanabe G, Yasui A, and Lieber MR. PALF acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3′-exonuclease and can participate in DNA end joining in a biochemical system. J Biol Chem. 286, 36368-36377, 2011.
24. Zlatanou A, Despras E, Braz-Petta T, Boubakour-Azzouz I, Pouvelle C, Stewart GS, Nakajima S, Yasui A, Ishchenko AA, Kannouche PL. The hMsh2-hMsh6 complex acts in concert with monoubiquitinated PCNA and Pol η in response to oxidative DNA damage in human cells. Mol Cell. 43, 649-662, 2011.
25. Lan L, Ui A, Nakajima S, Hatakeyama K, Hoshi M, Watanabe R, Janicki SM, Ogiwara H, Kohno T, Kanno SI, and Yasui A. The ACF1 complex is required for DNA double-strand break repair in human cells. Mol Cell 40, 976-987, 2010.
26. Ogiwara H, Ui A, Otsuka A, Satoh H, Yokomi I, Nakajima S, Yasui A, Yokota J, and Kohno T, Histone acetylation by p300 at double-strand break sites facilitates SWI/SNF chromatin remodeling and the recruitment of non-homologous end joining factors. Oncogene, 18, 2135-2146, 2011.
27. Horibata K, Saijo M, Bay MN, Lan L, Kuraoka I, Brook PJ, Honma M, Nohmi T, Yasui A, and Tanaka K. Mutant Cockayne syndrome group B protein inhibits repair of DNA topoisomerase I-DNA covalent complex. Genes Cells, 16, 101-114, 2011.
28. Itoh G, Kanno SI, Uchida KS, Chiba S, Sugino S, Watanabe K, Mizuno K, Yasui A, Hirota T, and Tanaka K. CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment. EMBO J. 30, 130-144, 2011.
29. Isogai S, Kanno S, Ariyoshi M, Tochio H, Ito Y, Yasui A, and Shirakawa M. Solution structure of a zinc-finger domain that binds to poly-ADP-ribose. Genes Cells 15, 101-110, 2010.
30. Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, and Wilson SH. DNA polymerase beta-dependent long patch base excision repair in living cells. DNA Repair, 9, 109-119, 2010.
31. Yasui A, Lan L, Nakajima S, Hatakeyama K, Zehui H, and Kanno SI. Repair of DNA strand breaks in living human cells and implications for cancer therapy. In “Extended Abstracts for the 40th International Symposium of the Princess Takamatsu Cancer Research Fund”, 68-73, 2010.
32. Mori H, Ouchida R, Hijikata A, Kitamura H, Ohara O, Li Y, Gao X, Yasui A, Lloyd RS, and Wang JY. Deficiency of the oxidative damage-specific DNA glycosylase NEIL1 leads to reduced germinal center B cell expansion. DNA Repair (Amst). 8,1328-1332, 2009
33. Ziv O, Geacintov N, Nakajima S, Yasui A, and Livneh Z. DNA polymerase zeta cooperates with polymerases kappa and iota in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients. Proc Natl Acad Sci U S A. 106, 11552-11557, 2009
34. Minami Y, Kasukawa T, Kakazu Y, Iigo M, Sugimoto M, Ikeda S, Yasui A, van der Horst GT, Soga T, and Ueda HR. Measurement of internal body time by blood metabolomics. Proc Natl Acad Sci U S A. 106, 9890-9895, 2009
35. Wei L, Lan L, Hong Z, Yasui A, Ishioka C, and Chiba N. Rapid recruitment of BRCA1 to DNA double-strand breaks is dependent on its association with Ku80. Mol Cell Biol. 28, 7380-7393, 2008.
36. Hong Z, Jiang J, Hashiguchi K, Hoshi M, Lan L, and Yasui A. Recruitment of mismatch repair proteins to the site of DNA damage in human cells. J. Cell Sci. 121, 3146-3154, 2008.
37. Niimi A, Brown S, Sabbioneda S, Kannouche P, Scott, A, Yasui A, Green CM, and Lehmann AR. Regulation of proliferating cell nuclear antigen ubiquitination in mammalian cells. Proc. Natl. Acad. Sci. USA, 105, 16125-16130, 2008
38. Petta TB, Nakajima S, Zlatanou A, Despras E, Sarasin A, Yasui A, and Kannouche P. Human DNA polymerase iota protects cells against oxidative stress. EMBO J. 27, 2883-2895, 2008.
39. Hong Z, Jiang J, Lan L, Nakajima S, Kanno S, Koseki H, and Yasui A. A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell. Nucleic Acids Res. 36, 2937-2947, 2008.
40. Honma S, Yasuda T, Yasui A, van der Horst GT, and Honma K. Circadian behavioral rhythms in Cry1/Cry2 double-deficient mice induced by methamphetamine. J Biol Rhythms. 23, 91-94, 2008.
41. Prasad R, Liu Y, Deterding LJ, Poltoratsky VP, Kedar PS, Horton JK, Kanno SI, Asagoshi K, Hou EW, Khodyreva SN, Lavrik OI, Tomer KB, Yasui A, and Wilson SH. HMGB1 is a co-factor in mammalian base excision repair. Mol. Cell, 27, 829-841, 2007.
42. Friedberg EC, Hanaoka F, Tanaka K, Wilson S, and Yasui A. The 3rd Japan-US DNA Repair Meeting, Sendai, Japan, May 7-11, 2007. DNA Repair, 6, 1545-1555, 2007.
43. Kamath-Loeb AS, Li L, Nakajima S, Yasui A, and Loeb LL. The Werner syndrome protein interacts functionally with translesion DNA synthesis. Proc. Natl. Acad. Sci. USA., 104, 10394-10399, 2007.
44. Kanno S, Kuzuoka H, Sasao S, Hong Z, Lan L, Nakajima S, and Yasui A. A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses, EMBO J. 26, 2094-2103, 2007.
45. Hashiguchi K, Matsumoto Y, and Yasui A. Recruitment of DNA repair synthesis machinery to sites of DNA damage/repair in living cells. Nucleic Acids Res. 35, 2913-2923, 2007.
46. Hirano M, Yamamoto A, Mori T, Lan L, Iwamoto TA, Aoki M, Shimada K, Furiya Y, Kariya S, Asai H, Yasui A, Nishiwaki T, Imoto K, Kobayashi N, Kiriyama T, Nagata T, Konishi N, Itoyama Y, and Ueno S. DNA single-strand break repair is impaired in aprataxin-related ataxia. Ann Neurol. 61,162-174, 2007.
47. Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Murakawa Y, Zeng W, Yokomori K, Helleday T, Teraoka H, Arakawa H, Buerstedde JM, and Takeda S. RAD18 and poly(ADP-ribose) polymerase independently suppress the access of nonhomologous end joining to double-strand breaks and facilitate homologous recombination-mediated repair. Mol Cell Biol. 27, 2562-2571, 2007.
48. Nakajima S, Lan L, Kanno S, Usami N, Kobayashi K, Mori M, Shiomi T, and Yasui A. Replication-dependent and −independent responses of RAD18 to DNA damage in human cells. J. Biol. Chem. 281, 34687-34695, 2006.
49. Jans J, Garinis GA, Schul W, van Oudenaren A, Moorhouse M, Smid M, Sert YG, van der Veide A, Rijksen Y, de Gruijl FR, van der Spek PL, Yasui A, Hoeijmakers JH, Leenen PJ, and van der Horst GT. Differential role of basal keratinocytes in UV-induced immunosuppression and skin cancer. Mol Cell Biol. 26, 8515-8526, 2006.
50. Yoshimura M, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde JM, Hochegger H, Okada T, Hiraoka M, and Takeda S. Vertebrate PolQ cells against oxidative DNA damage through base excision repair and translesion DNA synthesis. Mol Cell, 24, 115-125, 2006.
51. Karmakar P, Seki M, Kanamori M, Hashiguchi K, Ohtsuki M, Murata E, Inoue E, Tada S, Lan L, Yasui A, and Enomoto T. BLM is an early responder to DNA double-strand breaks. Biochem. Biophys. Res. Commun. 348, 62-69, 2006.
52. Hirano M, Furuya Y, Asai H, Yasui A, and Ueno S. ALADINI482S causes selective failure of nuclear protein import and hypersensitivity to oxidative stress in triple A syndrome. Proc. Natl Acad. Sci. USA. 103, 2298-2303, 2006.
53. Lan L, Nakajima S, Komatsu K, Nussenzweig A, Shimamoto A, Oshima J, and Yasui A. Accumulation of Werner protein at DNA double-strand breaks in human cells. J. Cell Sci. 118, 4153-4162, 2005.
54. Takao M, and Yasui A. DNA repair initiated by glycosylases in the nucleus and mitochondria of mammalian cells; how our cells respond to a flood of oxidative DNA damage. J. Dermat. Sci. Suppl. 1, S9-S19, 2005.
55. Lo H-L, Nakajima S, Ma L, Walter B, Yasui A, Ethell DW, and Owen LB. Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest. BMC Cancer, 5, 135-143, 2005.
56. Hamdi M, Kool J, Cornelissen-Steijger P, Carlotti F, Popeijus HE, van der Burgt C, Janssen JM, Yasui A, Hoeben RC, Terleth C, Mullenders LH, and van Dam H. DNA damage in transcribed genes induces apoptosis via the JNK pathway and the JNK-phosphatase MKP-1. Oncogene, 24, 7135-7144, 2005.
57. Braithwaite EK, Kedar PS, Lan L., Polosina YY, Asagoshi K, Poltoratsky V, Horton JK, Miller H, Teebor GW, Yasui A, and Wilson SH. DNA polymerase l protect mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. J. Biol. Chem. 280, 31641-31647. 2005.
58. Okano S, Lan L, Tomkinson AE, and Yasui A. Translocation of XRCC1 and DNA ligase IIIa from centrosomes to chromosomes in response to DNA damage in mitotic human cells. Nucleic Acids Res. 33, 422-429, 2005.
59. Moser J, Volker M, Kool H, Alekseev S, Vrieling H, Yasui A, van Zeelad AA, and Mullender LH. The UV-damaged DNA binding protein mediates efficient targeting of the nucleotide excision repair complex to UV-induced photo lesions. DNA Repair, 4, 571-582, 2005.
60. Wilson TM, Vaisman A, Martomo SA, Sullivan P, Lan L, Hanaoka F, Yasui A, Woodgate R, and Gearhart PJ. MSH2-MSH6 stimulates DNA polymerase h, suggesting a role for A-T mutation in immunoglobulin genes. J. Exp. Med. 201, 637-645, 2005.
61. Jans J, Schul W, Sert Y-G, Rijksen Y, Rebel H, Eker APM, Nakajima S, van Steeg H, de Gruijl FR, Yasui A, Hoeijmakers JHJ, van der Horst GTJ. Powerful skin cancer protection by a CPD-photolyase transgene. Curr. Biology, 15, 1-20, 2005.
62. Zhang QM, Yonekura S, Takao M, Yasui A, Sugiyama H, and Yonei S. DNA glycosylase activities for thymine residues oxidized in the methyl group are functions of the hNEIL1 and hNTH1 enzymes in human cells. DNA Repair, 4, 71-79, 2005.